Dino

Bes

Bes

Synonyms N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid; N,N-Bis(2-hydroxyethyl)taurine

CAS Number: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight: 213.248 g/mol

Beilstein Registry Number: 1781572 EC Number: 233-465-5 MDL Number: MFCD00007533

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SKU 02100927-CF
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Description

Product Description

Bes

Application Notes

The effect of BES and other zwitterionic buffers on the Bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.BES may be employed as binding buffer in modified Eagle's medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Buffer

Specifications
SKU 02100927-CF
Application Notes The effect of BES and other zwitterionic buffers on the Bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.BES may be employed as binding buffer in modified Eagle's medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.
Beilstein Registry Number 1781572
EC Number 233-465-5
Format Crystalline
Hazard Statements H315-H319-H335
Applications Buffer
Molecular Formula C6H15NO5S
Molecular Weight 213.248 g/mol
Personal Protective Equipment Dust mask ,Eyeshields, Gloves
Physical Appearance White Powder
pKa 7.1 at 25°C
Product Families Description BES
Product Overview Bes
Purity ≥99%
RTECS Number KI7363500
Safety Symbol GHS07
Storage and Handling Store at Room Temperature (15-30 °C)
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.