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Isoamyl Alcohol

Isoamyl Alcohol

Synonyms Isopentyl alcohol; 3-Methyl-1-butanol; 3-Methylbutanol; Isobutyl carbinol; Primary isoamyl alcohol; Fermentation amyl alcohol

CAS Number: 123-51-3 Molecular Formula: C5H12O Molecular Weight: 88.15 g/mol

Beilstein Registry Number: 1718835 EC Number: 204-633-5 MDL Number: MFCD00002934

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SKU 02155078-CF
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Description

Product Description

Isoamyl Alcohol

Application Notes

Isoamy alcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction. Isoamyl alcohol is added to the phenol solution to help inhibit RNase activity and to help prevent the solubilization in the phenol phase of long RNA molecules with long poly(A) portions. It will also help in reducing the foaming during the extraction process.Isoamyl alcohol is also used in the extraction of ethidium bromide from DNA solutions and in the radiolabeling of RNA transcripts in nuclei that have been isolated from tissue. Isoamy alcohol has various miscellaneous uses which include HPLC analysis of various pharmaceuticals and metabolites, analysis of oxidized and reduced pyridine nucleotides and adenylates in organic phenol extracts from mitochondria, isolation of mRNA from a thermophilic cyanobacterium, Solvent for fats, resins, alkaloids, Manufacturing isoamyl (amyl) compounds, isovaleric acid, mercury fulminate, pyroxylin, artificial silk, lacquers, smokeless powders, for dehydrating celloidin solutions, for determining fat in milk etc.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

DNA purification||Inhibit RNAse activity||Solvent for fats||Determining fat in milk|| HPLC analysis of pharmaceuticals

Specifications
SKU 02155078-CF
Application Notes Isoamy alcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction. Isoamyl alcohol is added to the phenol solution to help inhibit RNase activity and to help prevent the solubilization in the phenol phase of long RNA molecules with long poly(A) portions. It will also help in reducing the foaming during the extraction process.Isoamyl alcohol is also used in the extraction of ethidium bromide from DNA solutions and in the radiolabeling of RNA transcripts in nuclei that have been isolated from tissue. Isoamy alcohol has various miscellaneous uses which include HPLC analysis of various pharmaceuticals and metabolites, analysis of oxidized and reduced pyridine nucleotides and adenylates in organic phenol extracts from mitochondria, isolation of mRNA from a thermophilic cyanobacterium, Solvent for fats, resins, alkaloids, Manufacturing isoamyl (amyl) compounds, isovaleric acid, mercury fulminate, pyroxylin, artificial silk, lacquers, smokeless powders, for dehydrating celloidin solutions, for determining fat in milk etc.
Auto Ignition 662° F (USCG, 1999)
Beilstein Registry Number 1718835
Boiling Point 270° F at 760 mm Hg (USCG, 1999)
Density 0.81 at 68° F (USCG, 1999)
EC Number 204-633-5
Flash Point 114° F (USCG, 1999)
Format liquid
Hazard Statements H226-H332-H335; Supplemental Hazard Statements: Repeated exposure may cause skin dryness or cracking.
Applications DNA purification||Inhibit RNAse activity||Solvent for fats||Determining fat in milk|| HPLC analysis of pharmaceuticals
Melting Point -179° F (NIOSH, 2016)
Molecular Formula C5H12O
Molecular Weight 88.15 g/mol
Personal Protective Equipment Eyeshields, Faceshields, full-face respirator , Gloves, multi-purpose combination respirator cartridge respirator filter
Physical Appearance Clear; Colorless Liquid
Product Families Description Isoamyl alcohol
Product Overview Isoamyl Alcohol
Purity ≥99%
Refractive Index n20/D 1.406(Lit.)
RTECS Number EL5425000
Safety Symbol GHS02, GHS07
Solubility 2 % at 57° F (NIOSH, 2016)
Storage and Handling Store at Room Temperature (15-30 °C).
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Vapor Density 3.04 (AIR=1)
Vapor Pressure 28 mm Hg (NIOSH, 2016)