Dino

ISIS DNA POL (1U/uL)

ISIS DNA POL (1U/uL)

Synonyms High Fidelity Polymerase |Proofreading DNA Polymerase

EC Number: 232-741-2

Configure Your Product:

SKU 11EPSIS100-CF
Price:
$29.50
In Stock
Request a quote
Description

Product Description

Isis™ DNA Polymerase is a proofreading DNA polymerase and amplifies DNA with 40 times more accuracy than standard Taq DNA polymerase.

Application Notes

Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |High Fidelity PCR

Specifications
SKU 11EPSIS100-CF
Application Notes Ideal for high-fidelity amplification |Use to amplify DNA with difficult to read secondary structures |Optimal for blunt-end PCR cloning |The most thermostable polymerase available |Useful to amplify GC rich sequences
Brand Isis™
Certifications Free of RNases and endo- and exonucleases
Components 100 U Isis™ DNA Pol at 1 U/µL |Isis™ buffer 10x C (1ml) with MgSO4 |1 mL MgSO4 at 30 mM
Concentration 1 U/µl
EC Number 232-741-2
Format Kit
Grade Molecular Biology Grade
Applications Molecular Biology |PCR/Amplification |PCR |cDNA synthesis |RT-PCR |DNA labeling |High Fidelity PCR
Kit Compatibility Any DNA Isolation or Purification Kit
Kit or Assay Type PCR Kits
Molecule Enriched DNA
Personal Protective Equipment Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Enzyme and reagents
Presentation Kit
Product Families Description Isis™ is a proofreading DNA polymerase and amplifies DNA with 40 times more accuracy than standard Taq DNA polymerase. The error rate is 0.66 x 10-6 mutation frequency/bp/duplication as determined by the Flaman method. Isis DNA polymerase is isolated from Pyrococcus abyssi and is an excellent choice for applications requiring high fidelity, like error-free cloning. Isis DNA Polymerase does not require primer 3’-end protection, unlike many other proofreading enzymes. This saves time during reaction setup and optimization. Isis DNA polymerase is able to amplify up to 6 kb on genomic DNA templates and 18 kb on phage DNA under a variety of amplification conditions. Isis DNA polymerase offers robust activity and flexibility.
Product Overview Isis™ DNA Polymerase is the most thermostable proofreading polymerase available for high fidelity PCR. Isis DNA Pol Kit contains Isis DNA polymerase, optimized incubation buffers with and without MgCl2 and a separate MgCl2.
Protein or Enzyme Type Molecular Biology Enzymes
References (1) Flaman, J.M., et al. (1994) Nucl. Acids Res. 15, 3259-3260 |(2) Flaman, J.M., et al. (1995) Proc. Natl. Acad. Sci. USA 92, 3963-3967 |(3) Gueguen, Y. et al., (2001) Eur. J. Biochem. 268, 1-10 |(4) Gueguen, Y. et al., (2002) FEMS Microbiology Letters. 217, 89-94
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation |Soil Science |Proteomics |Metagenomics |Metabolomics |Food Sciences |Forensics |Plant Pathology |Communicable Disease |Cancer |Agricultural |Microbiomics |Epigenetics
Storage and Handling Store at -20 °C.
Unit Definition One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74 °C under assay conditions.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.